Welcome to our forums! Please introduce yourself here


#1

Hi Everyone!

Bonjour, Hola, 你好, Hallo, Ciao, Namaste, Привет, Vítejte, 안녕하세요, chào bạn, こんにち, Pozdrowienia, Hej, Živjo, 拝啓, Tervetuloa, Velkommen, Cześć, Oi, Saudações, ようこそ, Salve, مرحبا, Benvenuto, Pozdrav, Merhaba, Привет, Hei, Bemvindo, Hälsningar, Välkommen, Saluto, مرحبا, zdravo, Χαίρετε…

We’re really excited to launch this community. To get things started, here are a few questions for everyone to get acquainted with one another :smiley:

  • Who are you?
  • Are you new to PCR technology?
  • What is your industry and profession?
  • How does Chai’s products apply to your specific project goals?

For any customer service or technical support questions, please email us at support@chaibio.com


#2

#3

Hi,

My name is Laurie and I have a veterinarian office. I’m relatively new to pcr and am interested in detecting canine parvovirus. Is your instrument able to do this?

thanks!


#4

Hi Laurie,

Thanks for reaching out!

Open qPCR uses qPCR technology to identify the presence of your target nucleotide sequences. This differs from traditional PCR in the following ways:

  • Real-time monitoring of data
  • Quantitative data analysis
  • High sensitivity and specificity
  • High resolution
  • Large dynamic range for detecting low and high target copy numbers
  • Automated detection
  • No post-PCR processing such as a gel required
  • Can use both intercalating dyes and fluorophore probes for detection

To answer your question – yes, the instrument is able detect for Parvovirus in a sample provided that you design primers specific to that target of interest. To detect a certain sequence, you will need the following:

  1. Sample – Isolate DNA or cDNA
  2. Detection –
    a. Primers/probes specific to the marker you wish to amplify OR
    b. Primers specific to the marker you wish to amplify and fluorescent dye (such as SYBR Green)
  3. Reaction Components - PCR Master mix (polymerase, dNTPs, MgCl, etc.)

The instrument will essentially amplify the target marker into billions of copies that is subsequently read out as a fluorescent signal. You may use commercially available PCR assays for detecting your target or develop your own assay. Should you wish to design your own assay, there are primer designing tools that can assist you with the process. IDT’s PrimerQuest is a good place to start (https://www.idtdna.com/primerquest/Home/Index). You may also purchase your primers directly form IDT once your design is ready.

Let me know if you have any further questions.

Cheers!
Lily


#5

Hi,
My name is Jeff and I am new to PCR, you work at a creative and exciting project, hope get better and better.


#6

Hi Jeff,

Thanks for the support! :slight_smile: Let us know if you have any questions about PCR/qPCR.

Cheers,
Lily


#7

Hello Chai Community!

I’m Amy and I work for the Zoonotic Disease Program at Washington State Dept of Health. We are using the dual-channel Open QPCR to detect pathogens from environmental samples and insect vectors. I have nearly 10 years experience with PCR, but I have had an Open QPCR for only a few months. I also want to give kudos to the Chai support team; Lily has been very helpful!


#8

Thanks for sharing - we’re pleased to welcome you and hear about your application!

-Josh


#9

Hello Community!

I´m Tali Gutierrez, I work for a small company in Mexico (ACTG Molecular Solutions) developing detection systems for aquaculture pathogens. We are happy users of Open qPCR since 6 months ago, reliable, fast and cheapest way to make diagnosis.