I would like suggestions on the best way to setup the Open qPCR device for only one 30 minutes cycle at a constant temperature.
I managed to get the amplification as expected by setting one cycling stage with only one cycle at the desired temperature. However this approach is kind of end-point while I would like to get also the amplification curve.
Since the amplification curve is based on the number of cycles and not time, would it be feasible to define a certain number of stages, each of 1 cycle and then obtain the amplification curve as an aggregate? Has anyone used the device for a similar application?
Thanks in advance.