We just got our hands on an OpenQPCR machine and currently trying to calibrate it to give it a spin. So far,
- we have updated it to the most recent firmware: v220.127.116.11
- ran the heating-related diagnostics: https://cl.ly/1J0a280P3G08
- but failed at the optical diagnostics: https://cl.ly/3J0t2f3k2c45
Unfortunately, the calibration solution was lost during a recent move and therefore I ordered a fresh new ChaiGreen naively thinking that the calibration solution is just water + dye but using our 8-well strips with pseudo-calibration solutions in them (with 23.75 water + 1.25 20X dye) kept failing, where the poor Chai complains about the readings being too similar between wells. So I assume the calibration solutions have some DNA in them but I don’t know how much.
Do you still provide the calibration solution or is there a way for us to make a home-brew one by mixing some DNA/RNA into the solution? I hope the recipe is open.