Hello all. I have been tasked with developing a protocol for using the Chai OpenPCR machine to quantify NGS libraries. I am new to qPCR in general, and figured I would reach out here first so that perhaps I don’t have to reinvent the wheel. If anyone in this community has used this product in this way, I would love to know which reagents and general protocol you have used, and any other tips and tricks you have on lock down. Thank you in advance!
I would like to use the NEBNext Library Quantification Kit. It does not appear to contain ROX reference dye. Has anyone successfully used this kit on the CHAI Open qPCR machine?